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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This information outlines more doable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines further probable optimization of mobile and immunoassay parameters to ensure the very best final results are...
Antibodies bound to the blocking peptide no longer bind towards the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates within the G1-S section boundary and is particularly degraded as cells development through S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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